Squaw Vine (Mitchella repens) has a long history of use in traditional North American herbal medicine, particularly among Native American and early European settler communities. It has been primarily used as a uterine tonic to support women during pregnancy, childbirth, and postpartum recovery. The plant was often made into teas or tinctures believed to help tone the uterus, ease menstrual discomfort, and facilitate labor and delivery. Its inclusion in formulas known as "partus preparator" was common among 19th-century midwives and herbalists.

Despite its traditional uses, there is very limited modern scientific research on Squaw Vine. No high-quality clinical trials have evaluated its effectiveness or safety for supporting the vagina or reproductive system. Most contemporary herbal literature cites only anecdotal or historical reports rather than laboratory or clinical data. The plant contains compounds such as tannins, saponins, and glycosides, but their specific effects on human reproductive tissues have not been thoroughly studied. As a result, while the use of Squaw Vine to support the vagina and related reproductive functions is well-documented in folk and historical sources, scientific validation is lacking.