Vitamin B6, particularly in its active form pyridoxal 5'-phosphate (PLP), has some scientific basis for supporting mood regulation, including symptoms related to "worry" (which may overlap with anxiety). PLP is a coenzyme involved in the synthesis of neurotransmitters such as serotonin, dopamine, and gamma-aminobutyric acid (GABA), which are implicated in mood and anxiety regulation. Several studies have investigated the relationship between B6 levels and mental health. For example, a 2022 randomized controlled trial found that high-dose vitamin B6 supplementation modestly reduced self-reported anxiety in healthy adults compared to placebo, though the effect size was small. Additionally, low levels of vitamin B6 have been observed in people with mood disorders, suggesting a potential association.

However, while there is a plausible biochemical rationale and some emerging evidence, the number and quality of studies specifically addressing vitamin B6 supplementation for "worry" or anxiety are limited. Most guidelines do not recommend B6 as a primary treatment for anxiety disorders, and systematic reviews highlight the need for more robust, larger-scale trials. Therefore, the use of vitamin B6 for worry has some scientific support, but the evidence is currently limited and not definitive.